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2 edition of Three biologically significant globotriaosylceramide binding sites on the verotoxin 1 B subunit found in the catalog.

Three biologically significant globotriaosylceramide binding sites on the verotoxin 1 B subunit

Darrin James Bast

Three biologically significant globotriaosylceramide binding sites on the verotoxin 1 B subunit

implications in toxin action, pathogenesis of disease & vaccine design.

by Darrin James Bast

  • 197 Want to read
  • 19 Currently reading

Published .
Written in English


The Physical Object
Pagination222 leaves.
Number of Pages222
ID Numbers
Open LibraryOL18834780M
ISBN 100612351068

Bekri, O. Lidove, R. Jaussaud, B. Knebelmann, F. Barbey. "The role of ceramide trihexoside (globotriaosylceramide) in the diagnosis and follow-up of the efficacy of treatment of Fabry disease: a review of the literature". U3 Cholesterol is a component of animal cell membranes. A1 Cholesterol in mammalian membranes reduces membrane fluidity and permeability to some solutes. S1 Drawing of the fluid mosaic model. [Drawings of the fluid mosaic model of membrane structure can be two dimensional rather than three dimensional.

Globotriaosylceramide (Gb3) is a glycosphingolipid present in cellular membranes. This lipid is degraded in the lysosome through the action of the lysosomal enzyme α‐Galactosidase A (EC , α‐Gal A).Cited by: 8. 1) The protein folds to make itself as compact as possible. 2) The packing of the protein is such that hydrophilic residues appear on the surface where they can interact with an aqueous environment. 3) The fold allows for the formation of clefts, which are often where the chemical transformation catalyzed by that protein takes place.

Here is an example of a protein whose tertiary structure contains both a-helices and b-sheets, and various turns that connect them (this is an enzyme known as 2,5-diketo-D-gluconate reductase). The a -helices are represented in red, the b -sheets in cyan. The binding of Ca2+ to troponin C increases toponin C interaction with troponin I and decreases troponin I with ____ which makes myosin-binding site accesible: tropomyosin: The calcium pump consists of the following characteristics except: is a dodecamer: BPG lowerds the affinity for oxygen of hemoglobin by: binding preferentially to.


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Three biologically significant globotriaosylceramide binding sites on the verotoxin 1 B subunit by Darrin James Bast Download PDF EPUB FB2

Akira Makita, Naoyuki Taniguchi, in New Comprehensive Biochemistry, Galabiaosykeramide (digalactosylceramide, structure in Table ). This lipid, along with globotriaosylceramide (I-6 in Table ), was found by Sweeley and Klionsky [] to be one of the lipids stored in the kidneys of a patient with Fabry's disease, a genetic α-galactosidase deficiency [,].

All members of the verotoxin family bind to the neutral GSL, globotriaosyl ceramide, (GalαGalβGlcβceramide, Gb 3). 16 This glycolipid (Figure 1), also known as the B-cell differentiation antigen CD77, 17 and the blood group antigen p k, 18 is bound with high affinity by the receptor binding B.

globotriaosylceramide: (glō'bō-trī'ă-ō-sil-ser'ă-mīd), A sphingolipid containing three sugar moieties that accumulates in people with Fabry disease.

Synonym(s): trihexosylceramide. Molecular modeling, in combination with an analysis of the binding of these toxins to deoxyGb 3 analogues resulted in the identification of two potential Gb 3 binding sites per B subunit monomer [].One site (cleft site or site 1) was between adjacent B subunit monomers while the other was in a shallow depression on the B subunit pentamer surface which opposes the plasma membrane of the target Author: Murugesapillai Mylvaganam, Beth Binnington, Monique Budani, Anna M.

Soltyk, Clifford A. Lingwood. Any three of the following are acceptable. Bacterial cells contain a single RNA polymerase, whereas eukaryotic cells have three.

Bacterial RNA polymerase can initiate transcription without the help of additional proteins, whereas eukaryotic RNA polymerases need general transcription factors. A major outbreak caused by Escherichia coli of serotype OH4 spread throughout Europe in This large outbreak was caused by an unusual strain that is most similar to enteroaggregative E.

coli (EAEC) of serotype OH4. A significant difference, however, is the presence of a prophage encoding the Shiga toxin, which is characteristic of enterohemorrhagic E. coli (EHEC) by: •4 subunits (2 alpha, 2 beta), oxygen, tetramer •alpha helices most prevalent •each subunit has a set of alpha helices in the same arrangement as the helices in myoglobin; a and B chains related to each other and to myoglobin by divergent evolution (they are incredibly conserved and share the majority of their structure, especially in the proximal and distal histidines).

washed three times with 1× PBS and then probed with rabbit polyclonal anti-Stx2a (,) for 1 h at room temperature. Lastly, the sections were stained with goat anti-rabbit-Alexa Fluor (1. Shiga toxin B-subunit (STxB), a protein involved in the cell-binding and intracellular trafficking of Shiga holotoxin, binds to a specific glycolipid, the globotriaosyl ceramide (Gb(3)).

Solvent A comprised % FA/5% ACN and Solvent B % FA/ACN. A mobile-phase gradient was produced during a 25 min run: 0 min, 1% B; 10 min, 99% B; 16 min, 99% B; min, 1% B; and 25 min, 1% B. The flow rate was μL/min.

A Q-Exactive mass spectrometer (Thermo Fisher Scientific) was used for the detection of lyso-Gb3 and its by: 7. Gb 3 was not expressed in normal colonic epithelial cells (Fig. 1 A and B), nonmalignant colonic adenomas (Fig.

1C), or nonmetastatic primary colon cancers (Fig. 1D). In contrast, as a positive control, another GSL G M1, the cholera toxin B subunit receptor, was readily detectable on the apical surface of colonocytes (Fig.

1B).Cited by: Globotriaosylceramide accumulation in the Fabry kidney is cleared from multiple cell types after enzyme replacement therapy BETH L. THURBERG,HELMUT RENNKE,1 ROBERT B. COLVIN,1 STEVEN DIKMAN,1 RONALD E.

GORDON,D COLLINS,ROBERT J. DESNICK. Globotriaosylceramide, Gb3, is an alternative functional receptor for Shiga-like toxin 2e. Infect Immun. ; 63(3) (ISSN: ) Keusch GT; Jacewicz M. The pathogenesis of Fabry disease is poorly understood.

We used a variety of immunohistological techniques to localize globotriaosylceramide, the main glycolipid that accumulates in Fabry disease. Globotriaosylceramide immunoreactivity in a heterogenous pattern was present in all organs examined of a patient on long-term enzyme replacement by: downregulation by globotriaosylceramide (Gb3).

Cells were exposed to Gb3 for 24 hours. A and B, After incubation with Gb3, immunoblotting was performed using anti-K Ca antibody in mouse aortic endothelial cells (MAECs; A) or human umbilical vein endothelial cells (HUVECs; B).

The results have been normalized to the level of β-actin. Globotriaosylceramide (Gb(3)) is a glycosphingolipid present in the plasma membrane that is the natural receptor of the bacterial Shiga toxin. The unsaturation level of Gb(3) acyl chains has a drastic impact on lipid bilayer properties and phase behaviour, and on many Gb(3)-related cellular processes.

For example: the Shiga toxin B subunit forms tubular invaginations in the presence of Gb(3 Cited by:   All of the following soluble factors enhance isotype switching from IgM to IgA in B cells except _____. ic acid -9 c. -4 f.B-cell activating factor (BAFF) Answer: Which one of the following features renders all IgG antibodies less susceptible to proteolysis than the other antibody classes.

of the hinge region y [ ]. Globotriaosylceramide (Gb3) induces KCa downregulation in Fabry disease (FD). We investigated whether Gb3 induces KCa endocytosis and degradation.

KCa, especially plasma membrane-localized KCa, was downregulated in both Gb3-treated mouse aortic endothelial cells (MAECs) and human umbilical vein endothelial cells. Globular Proteins Most proteins which occur in the aqueous, intracellular environment or in the plasma are of globular nature: they are very approximately spherical in shape, or consist of several different lobes ().Generally, proteins with more than about amino acid residues are multi-domain (although there are exceptions eg 2CPP).The different domains of a single protein may be.

Introduction. Fabry disease (MIM ) is an X-linked recessive lysosomal storage disease caused by α-galactosidase A (Gla) deficiency, which leads to the accumulation of glycosphingolipids, particularly globotriaosylceramide (Gb3).

1 The incidence of Fabry disease (FD) has been estimated at 1 in 40 toand FD shows greater severity in males than in females. 1,2 In this disease Cited by:. Fabry disease is a lysosomal storage disorder caused by deficiency of α-galactosidase A (α-gal A), resulting in deposition of globotriaosylceramide (Gb3) in the vascular endothelium.

Deacylated Gb3, globotriaosylsphingosine (lyso-Gb3), also accumulated in the tissues of Fabry disease patients. Lyso-Gb3 has been suggested as a candidate : Yeo-Jin Jeon, Namhee Jung, Sung-Chul Jung. 1 INTRODUCTION. Fabry disease (FD, OMIM # ), is an X‐linked disease caused by variants in the GLA gene (GenBank reference sequence NG_), located at locus Xq, producing deficient hydrolysis activity of α‐galactosidase A enzyme (α‐Gal A; E.C.

), which leads to progressive lysosomal accumulation of globotriaosylceramide (Gb3, ChemIDplus ) and Author: Magdalena Cerón‐Rodríguez, Guillermo Ramón‐García, Edgar Barajas‐Colón, Isidro Franco‐Álvarez, Juan.Figure 1 delineates the growth rate of E. coli under the following growth conditions: (A) glycerol-only (B) lactose-only (C) glycerol-lactose, (D) glycerol-IPTG, and (E) glycerol-IPTG-lactose.

We observed that both the glycerol-only and glycerol-lactose systems exhibited faster growth rates than the lactose system. This decrease in the growthFile Size: KB.